8-amino-1-hydroxy - 13a - methyl-1h-cyclopenta [5,6] naphtho - 1,2 - g quinazolines and process



United States Patent Q 2,990,092 S-AMINO-l-HYDROXY 13a METHYL-lH-CYCLO- PENTA [5,6] NAPHTHO 1,2 g QUINAZOLINES AND PROCESS Frank B. Colton, Chicago, and Ivar Laos, Skokie, Ilh,

assignors to G. D. Searie & Co., Chicago, 111., a corporation of Delaware No Drawing. Filed Nov. 20, 1959, Ser. No. 854,238 7 Claims. (Cl. 260-2395) This invention relates to polyhydro 8-amino1-hydroxy- Ilia-methyl 1H cyclopenta[5,6]naphtho[1,2-g]quinazolines and' a process for the manufacture thereof.

011 A 1 '12 13a 2 3a 3 8 rim/W and A compounds otherwise identical, R in the formula being representative of hydrogen or a methyl radical, and R" representing hydrogen or a lower alkyl radical.

More particularly, this invention relates to chemical compounds of the formula 15 Among the lower alkyl radicals represented by R", methyl and ethyl radicals are preferred.

Equivalent to the foregoing compounds for purposes of this invention are non-toxic acid addition salts thereof, the composition of which may be depicted by wherein R and R" have the meanings previously assigned; X represents one equivalent of an anionfor example, chloride, bromide, iodide, nitrate, phosphate, sulfate, sulfamate, methyl sulfate, ethyl sulfate, benzenesulfonate, toluenesulfonate, acetate, lactate, succinate, malate, maleate, tartrate, citrate, gluconate, ascorbate, benzoate, cinnamate, or the likewhich, in combination with the cationic portion of a salt aforesaid, is neither 'pharmacologically nor otherwise undesirable in physiological dosage; and n is a small positive integer, optimal- The compounds to which this invention relates are useful because of their valuable pharmacological properties.

Especially, they are anabolic agents, and as such char- Patented Sept. 5, .1961

acterized by an unusual capacity to promote the retention of nitrogen in the animal body.

Manufacture of the basic amines of this invention proceeds by heating an appropriate 2-hydroxymethylenic steriod of the formula CHa (R and R being defined as before) with guanidine or a suitable salt thereof, for example, the carbonate, in an inert anhydrous solvent medium under a protective atmosphere. Conversion of the resultant base to a corresponding acid addition salt is accomplished by simple admixture of the contemplated base with at least one equivalent of any of various inorganic and strong organic acids, the anionic portion of which conforms to X as hereinabove defined.

The fundamental ring system characteristic of the instant compounds is named in accordance with recommendations of The Chemical Abstracts Service;

The following examples describe in detail compounds illustrative of the present invention, and methods which have been devised for their manufacture. However, the invention is not to be construed as limited thereby, either in spirit or in scope, since it will be apparent to those skilled in the art of organic synthesis that many modifications, both of materials and of methods, may be practiced without departing from the purpose and intent of this disclosure. temperatures are given in degrees centigrade and relative amounts of materials in parts by weight, except as otherwise noted.

EXAMPLE 1 8-amz'no 2,3,3a,3b,4,5,5a,6,11,11a,11b,12,13,13a-tetradecahydro-I-hydroxy-I1a,13a-dimethyl 1H cyclopenta- [5,61naphtho [1 ,Z-g] quinazoline.A solution of 520 parts of l7fl-hydroxy-Z-hydroxymethyleneandrostan-3-one and 157 parts of guanidine carbonate in 7800 parts of absolute ethanol protected by an atmosphere of nitrogen is heated at the boiling point under reflux for 20- hours, during which time a light beige precipitate is thrown down. The reaction mixture is then cooled to room temperatures, whereupon the precipitate is filtered'ofhwashed withpabi solute ethanol, and'dried. The resultant material isjsflus pended in 6000 parts of water.

at -95 with agitation for 5v minutes, then cooled to room temperatures and filtered. The material recovered on the filter is the desired 8-amino-2,3,3a,3b,4,5,5a,6,-l1,'

dimethyl' 1H cyclopenta[5,6jna h h 1,2;

line, characterized by absorption bands in the infra-red In the examples hereinafter set forth,

Thesuspensionisheated 3 spectrum at 2.88, 3.0, 3.2, 6.0, 6.2, and 6.4 a. The product has the formula CHI OBI

EXAMPLE 2 CH9 CH1 EXAMPLE 3 8-amin0 1 ethyl 2,3,3a,3b,4,5,5a,6,11,11a,1lb,12, 13,13a-tetradecahydr0 1 hydroxy 11a,13a dimethyl- IH cyclopenta[5,6]naphth0[1,2-g]quinazoline. Using the technique of Example 1, but substituting 570 parts of 17e-ethyl 1719 hydroxy 2-hydroxymethyleneandrostan- 3-one for the 520 parts of 17fl-hydroxy-2-hydroxymethyleneandrostan-3-one called for therein, one obtains S-amino-l-ethyl 2,3,3a,3b,4,5,5a,6,11,11a,11b,12,13,13a-tetradecahydro 1 hydroxy 11a,13adimethyl 1H cyclopenta[5,6]naphtho[1,2-g1quinazoline, characterized by absorption bands in the infra-red spectrum at 2.88, 3.0, 3.2, 6.0, 6.2, and 6.4;1. The product has the formula EXAMPLE 4 8-amin0 2,3,3a,3b,4,5,11,11a,1lb,12,13,13a doziecahydro 1 hydroxy 1,13a dimethyl 1H cyclopenta- [5,6]naphtho[1,Z-g]quinazoline.--Using the technique of Example 1, but substituting a like amount of 17fi-hydroxy- 2-hydroxymethylene 17cc methyl-l9-normethylandrost- 4-en-3-one for the l7fl-hydroxy-Z-hydroxymethyleneandrostan-S-one called for therein, one obtains S-amino- 2,3,3a,4,5,11,11a,l1b,12,13,13a dodecahydro 1 hydroxy 1,13a-dimethyl 1H cyclopenta[5,6]naphtho- [1,2-g] quinazoline, characterized by absorption bands in the infra-red at 2.88, 3.0, 3.2, 6.0, 6.2, and 6.4a. The product has the formula CHI EXAMPLE 5 8-amir10 2,3,3a,3b,4,5,11,11a,11b,12,13,13a dodecahydro 1 hydroxy 1,1la,]3a trimethyl 1H cyclopenta[5,6]naphtho[l,2-g]quinazoline.Using the technique of Example 1, but substituting 550 parts of 17;3-hydroxy-Z-hydroxymethylene 17m methylandrost 4-en-3- one for the 520 parts of 17fi-hydroxy-2-hydroxymethyleneandrostan-3-one called for therein, one obtains S-amino-2,3,3a,3b,4,5,11,11a,11b,12,13,13a dodecahydro-l-hydroxy 1,11a,13a tn'methyl 1H cyclopenta[5,6]naphtho[1,2-g] quinazoline, which, recrystallized from dimethylformamide, melts at 327-332 and exhibits absorption bands in the infra-red spectrum at 2.88, 3.00, 3.20, 6.01, 6.16, 6.27, and 6.42 1. The product has the formula CH: CH! iga and A compounds otherwise identical, R in the formula being selected from the group consisting of hydrogen and methyl radicals and R being selected from the group consisting of hydrogen and lower alkyl radicals.

2. 8 amino 2,3,3a,3b,4,5,5a,6,11,11a,11b,12,13,13atetradecahydro 1 hydroxy 11a,13a dimethyl 1H- cyclopenta[5,6]naphtho[1,2-g] quinazoline.

3. 8 -amino 2,3,3a,3b,4,5,5a,6,11,11a,1lb,12,13,13atetradecahydro 1 hydroxy 1,11a,13a trimethyl 1H- cyclopenta[5 ,6] naptho 1 ,2-g] quinazoline.

4. 8-amino 1 ethyl 2,3,3a,3b,4,5,5a,6,11,lla,11b, 12,13,13a -tetradecahydro 1 hydroxy 11a,13a-dimethy1-1H-cyclopenta[5,6] naphtho l,2-g] quinazoline.

5. S-amino 2,3,3a,3b,4,5,1l,l la,1-lb,12,13,13a-d0decahydro 1 hydroxy 1,13a-dimethy1 1H cyclopenta- [5 ,6] naphtho 1,2-g] quinazoline.

6. S-amino 2,3,3a,3b,4,5,11,11a,1'1b,12,13,13a-dodecahydro 1 hydroxy 1,11a,l3a trimethyl 1H cyclopenta[5,6]naphtho[1,2-g]quinazoline.

7. In a process for the manufacture of a compound 6 selected from the group consisting of compounds of the 0H. formula CH: on

OH R

H 110-0- R' j and A compounds otherwise identical, R in each of the foregoing formulas being selected from the group consisting of hydrogen and methyl radicals and R" in each of said formulas being selected from the group consisting of and A?" compounds otherwise identical, the step which 15 hydrogen and lower alky1radicals comprises vheating guamdine carbonate in an inert anhydrous solvent medium with a Z-hydroxymethylene steroid Reiel'ellces Cited in the file of this Patent selected from the group 3011585118 of compounds of the Fieser and Fieser, Steroids, Reinhold Pub. Corp.,

formula New York, 1959, page 1. 

1. A COMPOUND SELECTED FROM THE GROUP CONSISTING OF COMPOUNDS OF THE FORMULA 